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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Exp Cell Res. 2009 Sep 19;316(1):92–102. doi: 10.1016/j.yexcr.2009.09.015

Figure 1. Schematic presentation of the inducible system for expression of MORF4Flag in cultured cells.

Figure 1

MORF4 expression will be completely shut down without dox because the TRSID repressor complex (tTS) will recruit a mSin3/HDAC complex to this promoter. When Dox is added to culture medium, tTS will be replaced by tetracycline-controlled transactivator (rtTA) and rtTA will activate the promoter. LTR, long terminal repeat; SID, mSin3 interaction domain; PURO, puromycin resistant gene; CMV-P, cytomegalovirus promoter; HH4-P, histone H4 promoter; NEO, neomycin resistant gene; TRE, tetracyclin responsive element.