Figure 7. Cellular localization of MORF4 protein in HeLa cells.
HT-1 cells were treated with or without 1 µg/ml dox for 22 h and then treated with or without 10 µM MG132 for 2 h. Cytoplasmic and nuclear lysates were prepared and MORF4 and MRG15 proteins in the fraction detected by Western blot. Sp1 and α-tubulin were used as a loading control for nuclear and cytoplasmic fractions, respectively. The protein amount for loading was adjusted by cell number. The location of molecular markers is indicated (kDa). C; cyctoplasmic fraction and N; nuclear fraction.