Table 2.
Strong nuclear protein staining of CXCR4 is associated with decreased patient survival
| Patient characteristics | CXCR4 expression | Relation CXCR4 to: | Disease Free Survival | Overall Survival | |||||
|---|---|---|---|---|---|---|---|---|---|
| M-W | Univariate analysis | Multivariate analysis | Univariate analysis | Multivariate analysis | |||||
| Strong N = 43 | Weak N = 15 | p-value | HR (95% CI) | p-value | p-value | HR (95% CI) | p-value | ||
| Gender | |||||||||
| Male (%) | 21 (49%) | 7 (47%) | 0.9 | 0.8 | 0.9 | ||||
| Female (%) | 22 (51%) | 8 (53%) | |||||||
| Location tumor | |||||||||
| Proximal (%) | 21 (49%) | 10 (67%) | 0.2 | 0.6 | 0.7 | ||||
| Distal (%) | 22 (51%) | 5 (33%) | |||||||
| Median age at diagnosis (years) | |||||||||
| <69.7 | 21 (49%) | 8 (53%) | 0.8 | 0.008 | 2.5 | 0.01 | 0.006 | 2.8 | 0.008 |
| >69.7 | 22 (51%) | 7 (47%) | (1.2–4.9) | (1.3–5.8) | |||||
| TNM stage | |||||||||
| I and II | 28 (65%) | 11 (73%) | 0.6 | 0.002 | 2.9 | 0.003 | 0.002 | 3.3 | 0.002 |
| III | 15 (35%) | 4 (27%) | (1.4–5.8) | (1.5–6.8) | |||||
| Pathway | |||||||||
| MSI | 7 (16%) | 5 (33%) | 0.2 | 0.7 | 0.6 | ||||
| MSS | 36 (84%) | 10 (67%) | |||||||
| CXCR4 | |||||||||
| Strong | 0.07 | 2.6 | 0.04 | 0.03 | 3.7 | 0.02 | |||
| Weak | (1.0–6.2) | (1.35–11) | |||||||
Clinicopathological characteristics and survival results of patients with high and low nuclear protein expression of CXCR4. Level of CXCR4 was determined in an independent panel colorectal cancer patients. The table displays the results after immunohistochemical staining and semi-quantitative analyses of nuclear expression of CXCR4 in tumor cells, as described in materials and methods. For nuclear CXCR4 staining, 15 tumors were classified as low (26%) and 43 were strong (74%). On the left side of the table the distribution of high versus low expression of CXCR4 with respect to clinical and pathological characteristics and the relation of CXCR4 to clinicopathological factors are displayed. On the right side of the table, prognostic factors are displayed. Univariate Cox regression analyses were performed to identify prognostic factors for disease free and overall survival. All factors with a p value ≤ 0.10 were subjected to Multivariate Cox regression analysis. Numbers (N) of patients are indicated with percentages shown in parentheses
MSS microsatellite stable; MSI microsatellite instable; HR Hazard Ratio; CI Confidence Interval
aStatistical significant p-values are in bold