Table 4.
PhIP Content in Human Hair and Mouse Fur and Binding as a Function of Melanin Content
| Subject | Hair Color | Melanin content (mg/g hair) | A650/A500 | PhIP (pg/g hair) | PhIP (ng/g melanin) | |
|---|---|---|---|---|---|---|
| Vegetarian 1 | Light brown | 8.2 | 0.27 | <50 | N.D. | |
| Vegetarian 2 | Brown | 11.8 | 0.27 | <50 | N.D. | |
| Vegetarian 3 | Light brown | 7.4 | 0.20 | <50 | N.D. | |
| Vegetarian 4 | Dark brown | 12.8 | 0.28 | <50 | N.D. | |
| Vegetarian 5 | Light blonde | 8.3 | 0.08 | <50 | N.D. | |
| Vegetarian 6 | Black | 38.6 | 0.28 | 65 ± 5.8 | 1.7 ± 0.2 | |
| Meat-Eater 1 | Black-gray | 14.4 | 0.37 | 890 ± 40 | 64 ± 2.8 | |
| Meat-Eater 2 | Brown | 14.0 | 0.32 | 520 ± 21 | 37 ± 1.5 | |
| Meat-Eater 3 | Brown - red | 12.0 | 0.32 | 290 ± 14 | 24 ± 1.2 | |
| Meat-Eater 4 | Black | 54.8 | 0.33 | 490 ± 120 | 9.1 ± 2.2 | |
| Meat-Eater 5 | Brown | 12.5 | 0.32 | 790 ± 15 | 63 ± 1.2 | |
| Meat-Eater 6 | Black | not assayed | not assayed | 430 ± 27 | not assayed | |
| Mouse 1 | Black | 80.0 | 0.30 | 750 ± 65 | 9.4 ± 0.8 | |
| Mouse 2 | Black | 88.0 | 0.33 | 770 ± 75 | 8.8 ± 0.9 | |
| Mouse 3 | Black | 90.0 | 0.34 | 710 ± 165 | 7.9 ± 1.8 | |
| Mouse 4 | Black | 83.0 | 0.36 | 680 ± 180 | 8.2 ± 2.2 |
n = 3 independent measurements, mean ± SD
Mice ingested PhIP in contaminated chow (144 ng/kg body wt); humans estimated to ingest PhIP at 5.5 ng/kg body wt (40).
N.D. = Not detected