Skip to main content
. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Exp Cell Res. 2009 Sep 29;316(2):232–244. doi: 10.1016/j.yexcr.2009.09.021

Figure 5. Role of Rho kinase and myosin II in cell clustering.

Figure 5

Fibroblasts were cultured 16 hr on 1.0 mg/ml collagen matrices in PDGF or serum (FBS) media as indicated. Other additions were 10 μM Y27632 (Y) (Rho kinase inhibitor) or 20 μM blebbistatin (Bleb) (myosin II inhibitor). For FBS + charcoal, serum was treated with two rounds of activated charcoal. At the end of incubations, samples were fixed and stained for actin and with propidium iodide. Fibroblasts remained dispersed in PDGF medium, in medium containing charcoal-treated serum, and in serum medium with Rho kinase or myosin II inhibitors added. Scale bar, 100 μm.