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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Exp Cell Res. 2009 Sep 29;316(2):232–244. doi: 10.1016/j.yexcr.2009.09.021

Figure 7. PDGF stimulated migration is not inhibited by blocking myosin II.

Figure 7

Fibroblasts were cultured 16 hr on 1.5 mg/ml collagen matrices in PDGF media with 20 μM blebbistatin added as indicated. Cell migration was recorded by time-lapse microscopy. Randomly selected individual cell migration tracks were copied and combined into a single figure. Persistence of directional migration was determined morphometrically by measuring D/T ratios (direct distance from start to end point [D] divided by the total track distance [T]). Addition of blebbistatin caused cells to exhibit abnormally long dendritic extensions (arrows) but had little effect on overall cell migration in PDGF medium (Supplemental Video 3). Scale bar, 100 μm.