Figure 8. Reversibility of cell clustering patterns.
Fibroblasts were cultured for an initial period of 6 hr on 1.0 mg/ml collagen matrices in PDGF and serum media. Subsequently, the samples were rinsed and placed into PDGF and serum media as indicated for an additional 18 hr. At the end of the initial 6 hr period and after 24 hr, samples were fixed and stained for actin and with propidium iodide (PI). Morphogenetic cell patterns in PDGF and serum media were completely reversible. Scale bar, 100 μm.