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. Author manuscript; available in PMC: 2010 Dec 10.
Published in final edited form as: J Neuroimmunol. 2009 Oct 28;217(1-2):51–54. doi: 10.1016/j.jneuroim.2009.09.020

Figure 1.

Figure 1

Indicated amounts of human neutrophil lysate (106 cells) were assayed for PE enzymatic activity (expressed as mU/ml). Results are presented as mean ± SEM (A) and compared using the two group t test. Increased PE activity was observed with greater amounts of neutrophil lysate (* p < 0.05 compared with 0μl lysate, n = 3 per group). Results are representative of several experiments. Cytospins of human peripheral blood neutrophils on glass slides were incubated with anti-PE antibody (B), anti-PE antibody pre-adsorbed with PE (C) or pre-immune rabbit antibody (D), followed by FITC-labeled goat anti-rabbit IgG, and examined by immunofluorescence microscopy. PE in human neutrophils was located in the cytoplasm in a granular pattern (B).