Fig. 1.

Specificity of primers used for the identification of hGSTA5 transcript by RT-PCR. The PCR primers (shown in the bottom line of each panel) were designed so that they do not recognize targets other than Alpha-class GSTs (as shown by a BLAST search against the human genome; not shown), and that, among human Alpha class sequences, their 3′ end are unique for hGSTA5. A partial alignment of all human Alpha-class GSTs is depicted; nucleotides unique for only one of the five sequences are shown on white background, whereas nucleotides shared by two or more sequences are on shaded background. The numbering is relative to the translation initiation site. Panel A: primer 5F; panel B: 3F; panel C: 4F; panel D: 3R (the primer sequences are listed in Table 1).