Table 1.

Sequences of PCR primers used in the present work

Primer name	Primer sequence
1F	5′-TCCAGGAGACTgctagcATGGCAGAGAAGC
2F	5′-TcatatgGCAGAGAAGCCCAAGCTCCACTACTC
3F	5′-GGCTGCAGCTGGAGTAGAGTTG
4F	5′-CAAGACTACCTTGTTGGCAACAAGCTGAGCT
5F	5′-CTTTTCTACTACGTGGAAGAGCTTGACTCG
1R	5′-ATTggtaccGCATGTTCTTGGCCTCCATAGC
2R	5′-AggatccTCTGGCATGTTCTTGGCCTCCATAGCTGCT
3R	5′-AGCAGAGGGAAGCTGGAGATAAGACTC

Restriction enzyme recognition sites not present in the native sequence but introduced in the primer are shown in lower-case letters. Residues mutated to introduce these restriction sites are in bold. Regions of primers that correspond to protein coding sequences are underlined. Names of forward and reverse primers contain the letter F and R, respectively.