. Author manuscript; available in PMC: 2011 Jan 1.

Published in final edited form as: Biochim Biophys Acta. 2009 Aug 4;1800(1):16–22. doi: 10.1016/j.bbagen.2009.07.025

Table 2.

Catalytic activities of hGSTA5-5 expressed in E. coli ^a

Substrate	Specific activity (μmol/mg · min)^b

	Purification 1	Purification 2
1-chloro-2,4-dinitrobenzene	0.55 ± 0.07	0.64 ± 0.06
4-hydroxynonenal	7.63 ± 0.35	7.32 ± 0.45
trans-nonenal	3.67 ± 0.52	3.58 ± 0.67
acrolein	0.11 ± 0.05	0.05 ± 0.03
crotonaldehyde	ND	ND
cumene hydroperoxide	ND	ND

^a Activity was measured after solubilization/refolding and purification by affinity chromatography.

^b Results from two independent bacterial expression and purification experiments are shown. Data are given as means ± standard deviation. ND: not detectable.