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Catalytic activities of total GST fractions obtained from human HEK-293 cells transfected with hGSTA5(genomic)/pcDNA 3.1(-) and from untransfected control HEK-293 cells
| Cells | Specific activity (μmol/mg · min) | |
|---|---|---|
| 4-HNE | 1-chloro-2,4-dinitrobenzene | |
| Experiment 1 | ||
| HEK-293 | 1.32 ± 0.06 | 19.13 ± 0.05 |
| HEK-293/hGSTA5 | 2.13 ± 0.11 | 20.36 ± 0.08 |
| Experiment 2 | ||
| HEK-293 | 1.32 ± 0.52 | |
| HEK-293/hGSTA5 | 2.77 ± 0.83 | |
Total GST fractions were obtained by glutathione agarose affinity chromatography.
