. Author manuscript; available in PMC: 2011 Jan 1.

Published in final edited form as: Biochim Biophys Acta. 2009 Aug 4;1800(1):16–22. doi: 10.1016/j.bbagen.2009.07.025

Table 4.

Supplementation of an in vitro transcription/translation system with hGSTA5 cDNA results in increased catalytic activity for 4-HNE conjugation

Plasmid	Specific activity of 4-HNE conjugation (μmol/mg · min)
Plasmid	Insert-free pET-30a(+)	0.076 ± 0.007
Control plasmid ^a	0.079 ± 0.009
hGSTA5(cDNA)/pET-30a(+)
Reaction 1 ^b	0.133 ± 0.002
Reaction 2 ^b	0.114 ± 0.027
Reaction 3 ^b	0.102 ± 0.010
Reaction 4 ^b	0.106 ± 0.015
hGSTA5(cDNA)/pET-30a(+); protein product purified ^c	4.89 ± 1.67

^a Control plasmid supplied with the Qiagen EasyXpress Insect Kit II.

^b Four independent in vitro protein synthesis reactions were carried out and assayed for 4-HNE-conjugating activity without purification.

^c Pooled material from four independent reactions (see preceding note) was purified by glutathione agarose affinity chromatography.