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. Author manuscript; available in PMC: 2010 Dec 10.
Published in final edited form as: Vaccine. 2009 Oct 12;28(1):140–147. doi: 10.1016/j.vaccine.2009.09.086

Figure 4. T cell help but not a germinal center reaction is needed for the production of VacV neutralizing IgM.

Figure 4

(A) VacV specific IgG titers in WT and SAP−/− mice at days 8 (left) and 45 (right) post-infection (B) Anti-VacV neutralizing antibody titers in WT and SAP−/− mice at day 8 post-infection. Total (white bars) and IgM depleted sera (black bars) is shown. N = 6 for both groups. Error bars represent SEM. Dotted line indicates LOD. Data is representative of 2 independent experiments. (C) Flow cytometry of germinal center B cells from WT and SAP−/− mice at day 8 after VacV infection. B220+ gated cells are shown. Germinal center B cells are FashiGL7+. (D) Quantitation of germinal center B cells (B220+FashiGL7+), as a percentage of total splenic B cells. n = 4/group. Data are representative of three independent experiments.