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. Author manuscript; available in PMC: 2010 Dec 11.
Published in final edited form as: J Mol Biol. 2009 Sep 24;394(4):747–763. doi: 10.1016/j.jmb.2009.09.049

Fig. 2.

Fig. 2

Representative isothermal titration calorimetry measurements and fit: n-phenylglycinonitrile titration (stock concentration 1.4mM) into L99A/M102Q (initial concentration 42.5μM). An initial injection of 2μL was followed by 29 ×10μL injections of ligand to a final ligand concentration in the reaction cell of 237.9μM.

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