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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cell Microbiol. 2009 Aug 20;11(12):1827–1841. doi: 10.1111/j.1462-5822.2009.01374.x

Figure 7.

Figure 7

Metacyclic promastigotes co-localize with caveolin-1 and with CR3, but not MR. Purified metacyclic promastigotes were labeled with CFSE (green) and incubated with adherent MDMs at an MOI of 10:1. Coverslips were fixed at 2, 15, 30, 60 or 100 minutes after synchronized phagocytosis. Shown are the 30 and the 100 minute time points. CR3 or MR was labeled with anti-human CR3 or anti-human MR primary antibodies respectively, and Alexa Fluor 568 anti-mouse IgG secondary antibodies (red). Caveolin-1 was labeled with polyclonal anti-human caveolin primary and Alexa Fluor 647 anti-rabbit IgG secondary antibodies (blue). Parasites that clustered with caveolin-1 also accumulated CR3, but not MR (arrows, 30 min). Internalized parasites lost most of their caveolin staining (arrows) by 100 min after phagocytosis. Photomicrographs are representative of more than 5 experiments.