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Regulation of WUS expression patterns by various levels of auxin in a pWUS::GUS marker line. (a, b) PSEs cultured in liquid medium for 22 days. (c–h) PSEs cultured in ECIM for 14 days, and then cultured in SEIM for 8 days (total 22 days). (a, b) Medium without 2,4-D. (c, d) Medium with 1 μm 2,4-D. (e, f) Medium with 9 μm 2,4-D. (g, h) Medium with 18 μm 2,4-D. Scale bars = 1.2 mm.
