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. 2009 May 7;59(3):448–460. doi: 10.1111/j.1365-313X.2009.03880.x

Figure 6.

Figure 6

Auxin distribution marked by DR5::GUS expression. (a) DR5::GUS signals are regionally distributed on embryonic calli cultured in ECIM for 14 days. (b) Strong GUS signals (arrowheads) in somatic pro-embryos are seen on the inside edges of embryonic calli in SEIM at 2 days. (c) GUS signals are detectable in the apical domain of cotyledon primordia (arrowheads). (d) GUS signals accumulate in cotyledons (black arrowhead) and root (white arrowhead) poles of mature somatic embryos. EC, embryonic calli. Scale bars = 0.8 mm (a, b) and 1 mm (c, d).