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. 2009 Aug 11;17(12):2088–2095. doi: 10.1038/mt.2009.184

Figure 5.

Figure 5

Enhanced transduction of retinal Müller glia in vivo by ShH13. Representative images (original magnification ×25) of subretinal injections with dsCAG-GFP vectors with capsids from ShH13 and AAV6 reveal a pronounced shift in viral tropism toward Müller glia in the ShH13 variant compared to the parent AAV6 vector: GFP expression (green), overlay with GS+ Müller glia (red), isolation of areas of colocalization (yellow). The corresponding x-z and y-z planes are shown on the bottom and on the right side, respectively, for each image. Bar = 30 µm. (a) AAV6 expression is predominantly seen in photoreceptors and RPE (not shown), whereas (b) ShH13 shows robust expression in Müller glia and RPE (not shown). AAV, adeno-assocaited virus; RPE, retinal pigment epithelium.