Table 1.
Escherichia coli K-12 strains.
| Strain | Relevant Genotypea | Source |
|---|---|---|
| General P1 donors | ||
| N3793 | ΔrecG263::kan | Al-Deib et al. (1996) |
| RUC663 | tnaA::Tn10 dnaA46 | Tove Atlung |
| MG1655 and derivativesb | ||
| MG1655 | F–rph-1 | Bachmann (1996) |
| AM1655 | ΔrecG::apra | R.G., Lloyd and A.A. Mahdi, unpublished |
| APS345 | attTn7::lacO240::kan zdd/e::tetO240::gen | Rudolph et al. (2007) |
| AU1054 | tnaA::Tn10 dnaA46 | MG1655 × P1.RUC663 to Tcr |
| AU1091 | tnaA::Tn10 dnaA46ΔrecG263::kan | AU1054 × P1.N3793 to Kanr |
| N4560 | ΔrecG265::cat | Mahdi et al. (2006) |
| N5466 | ΔruvC::cat | Mahdi et al. (2006) |
| N5500 | priA300 | Jaktaji and Lloyd (2003) |
| RCe29 | priA300ΔrecG265::cat | Rudolph et al. (2009) |
| RCe72 | attTn7::lacO240::kan zdd/e::tetO240::genΔrecG265::cat | Rudolph et al. (2009) |
| RCe79 | dnaC7 | Rudolph et al. (2007) |
| RCe108 | priA300 attTn7::lacO240::kan zdd/e::tetO240::gen | Rudolph et al. (2009) |
| RCe109 | priA300 attTn7::lacO240::kan zdd/e::tetO240::genΔrecG265::cat | Rudolph et al. (2009) |
| RCe111 | dnaC7ΔrecG265::cat | Rudolph et al. (2009) |
| RCe113 | dnaC7ΔruvC::cat | RCe79 × P1.N5466 to Cmr |
| RCe197 | attTn7::lacO240::kan zdd/e::tetO240::gen tnaA::Tn10 dnaA46 | Rudolph et al. (2009) |
| RCe198 | attTn7::lacO240::kan zdd/e::tetO240::genΔrecG265::cat tnaA::Tn10 dnaA46 | Rudolph et al. (2009) |
| RCe246 | attTn7::lacO240::kan zdd/e::tetO240::gen tnaA::Tn10 dnaA46ΔrecG::apra | RCe197 × P1.N6052 to Aprar |
a
The abbreviations kan, cat, gen and apra refer to insertions conferring resistance to kanamycin (Kmr), chloramphenicol (Cmr), gentamicin (Genr) and apramycin (Aprar) respectively. Tn10 confers resistance to tetracycline (Tcr). Strains carrying dnaA46 or dnaC7 are temperature sensitive for growth. CGSC: Coli Genetic Stock Center, Yale University.
b
Only the relevant additional genotype of the derivatives is shown.