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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Placenta. 2009 Nov 5;30(12):1045–1051. doi: 10.1016/j.placenta.2009.10.007

Figure 4.

Figure 4

Effects of PD98059 and LY294002 on FGF2- and VEGF-induced phosphorylation of ERK1/2 and AKT1 in HPAE cells. Cells were cultured under normoxia for 24 hr. After serum starvation, cells were treated with FGF2 (A) for 5 min or VEGF (B) for 10 min in the absence or presence of PD98059 (20 μM) or LY294002 (5 μM) (1 hr pretreatment). Proteins were subjected to Western blot analysis for total ERK1/2 (tERK1/2) and total AKT1 (tAKT1) or phospho-ERK1/2 (pERK1/2) and phospho-AKT1 (pAKT1). Data are expressed as means ± SEM fold of the control (no growth factor and kinase inhibitor; n = 4). a,b,c,d Means with different letters differ (p < 0.05).