Skip to main content
. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Anal Biochem. 2009 Oct 2;396(2):212–216. doi: 10.1016/j.ab.2009.09.049

Figure 1.

Figure 1

Distribution of subcellular markers. Cell lysates (CL, 15μg) and plasma membrane enriched fractions (PM, 5μg) were separated by SDS-PAGE on a 10% gel and transferred to a PVDF membrane. Subcellular markers were detected using specific antibodies and an HRP-conjugated secondary antibody. Bands were visualized using Super-Signal West Femto Chemiluminescent Substrate and exposure in the SynGene Gnome. CL, cell lysate, PM, plasma membrane fraction.