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Dose-response curves demonstrating that replicate injections of various plasma membrane concentrations, randomly run, gave similar binding responses. Inset shows a plot of the replicate values obtained for each concentration of ricin used (1000, 500, 250, 125, and 62.5 nM). It can be seen that adherence of ricin to immobilized membranes did not change over time, or with repeated regeneration steps.
