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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Exp Cell Res. 2009 Aug 26;316(2):172–180. doi: 10.1016/j.yexcr.2009.08.011

Figure 6. Effect of DNMT inhibition by aza-dC on expression of inducers of endothelial cell differentiation.

Figure 6

The ESC were exposed to vehicle or 1 μM or 3 μM aza-dC for 24 hours in ESGRO COMPLETE™ basal media lacking LIF. Cultures were returned to basal medium without aza-dC after 24 h of treatment and cells were collected after 3, 7, and 15d of treatment. mRNA expression of the EC inducer genes (A) BMP4, (B) EPAS-1 and (C) VEGF was determined by quantitative real time PCR. Values are normalized over those of GAPDH (n=6). ** indicates a pvalue ≤0.01, for vehicle- vs. aza-dC-treated cells (ESC vs. vehicle- and aza-dC-treated cells).