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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cell Signal. 2009 Sep 26;22(2):221–233. doi: 10.1016/j.cellsig.2009.09.017

Fig. 7.

Fig. 7

Gγ-SaaX expression specifically within the PGCs results in migration defects that can be reversed by injection of one of several Gγ-WT mRNAs. Overexpression of gng3-WT [300pg mRNA injection] specifically in the PGCs has no effect on either somatic development as assayed by bright-field microscopy (A) or PGC migration as revealed in the corresponding fluorescent image (B). gng3-SaaX expression [150pg] specifically in the PGCs also has no effect on somatic development (C) but profoundly disrupts PGC migration (D). The effect of gng2-SaaX on PGC migration is reversed by injection with PGC-driven gng2-WT mRNA [200pg] (E & F) but not PGC-driven gng5-WT mRNA [550pg] (G & H). All embryos shown were injected at the 1 – 4 cell stage with mRNAs as described above, together with GFP-nos1-3′UTR mRNA and subjected to microscopy at 48hpf.