Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Dev Biol. 2009 Oct 19;337(1):110–123. doi: 10.1016/j.ydbio.2009.10.016

Fig. 2.

Fig. 2

The hatch rate of embryos provides a measure for retained function of Axin mutant proteins. Constitutive expression of Axin mutant proteins using the tubulin promoter (tub>Axin*) in axinnull embryos revealed that the point mutations in the Armadillo binding domain result in complete loss of function, equivalent to AxinΔArm. All or nearly all embryos expressing the point mutations in the Shaggy binding domain, AxinL447Q or AxinL447S, die prior to hatching and therefore behave similarly to deletion of the entire site (AxinΔSgg). In contrast, AxinK446E allows hatch rates at similar levels to expressed FLAxin, though animals subsequently die (in K446E). Hatch rates are FLAxin 80% ±0.3, n=554; AxinΔArm, 33.4% ±3.7; AxinL501P, 54% ±5.3, n=599; AxinH504L, 62% ±5.0, n=781; AxinΔSgg, 0% ±0, n=630; AxinK446E, 83% ±2, n=738; AxinL447S, 21.0%±13.7, n=857; AxinL447Q, 0%±0; n=520.