Fig. 2.

Gelatinolytic activity of MMP-2 of PMA-exposed HUVEC treated with 1-25 µg/mL tAgR (A) and tZj (B). After HUVEC culture protocols with 50 ng/mL PMA and 1-25 µg/mL tAgR and tZj for 24 h, equal volumes of culture media were subjected to 10% SDS-PAGE for the measurement of the enzyme activity of MMP-2. Gelatinolytic activity was detected as unstained bands against the background of Coomassie blue-stained gelatin. The bar graphs (means ± SEM, n=4) represent quantitative densitometric results of upper bands. ^*P<0.05, compared to untreated control. ^†P<0.05, compared to untreated control and 50 ng/mL PMA.