Fig. 1.
mRNA expressions of adipokines in BBR-treated 3T3-L1 adipocytes. At day 7 after inducing differentiation, adipocytes were cultured in 6-well plates with or without BBR for 18 hours. (a) Graph reporesents mRNA expressions of adipokines such as leptin, MCP-1, and adiponectin. Time course treatment berberine was conducted for 1 hour, 2 hours, 4 hours, 6 hours, 12 hours, and 24 hours. Graphs represent mRNA expressions of (b) TNF-α and (c) leptin. (*p<0.05, **p<0.01, ***p<0.001) Data are expressed relative to untreated control cells and represent means ± S.E. Experiments were conducted twice, each included triplication.