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. Author manuscript; available in PMC: 2010 Apr 29.
Published in final edited form as: Neuron. 2009 Oct 29;64(2):213–226. doi: 10.1016/j.neuron.2009.08.017

Figure 4. Palmitoylation of NR2A and NR2B by the palmitoyl acyl transferase GODZ.

Figure 4

(A) Palmitoylation of NR2A C-terminus in NR1/NR2A and NR1/NR2A 3CS by cotransfected palmitoyl acyl transferase GODZ or its inactive mutant GODZ(DHHS). (B) Palmitoylation of NR2A in NR1/NR2A and NR1/NR2A 4CS by cotransfected GODZ or GODZ(DHHS). (C) Palmitoylation of NR2A in NR1/NR2A and NR1/NR2A 7CS by cotransfected GODZ or GODZ(DHHS). (D) Palmitoylation of NR2B in NR1/NR2B, NR1/NR2B 3CS, NR1/NR2B 5CS and NR1/NR2B 8CS by cotransfected GODZ or GODZ(DHHS). Transfected HEK 293T cells were labeled with [3H]-palmitate and were then immunoprecipitated with anti-NR2A or anti-NR2B antibodies. The incorporated [3H]-palmitate signals were shown (upper top). Immunoprecipitation (IP, upper bottom) and total protein expression (cell lysate) of each protein (lower panels) were confirmed by western blotting (WB).