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. 2009 Dec 14;4(12):e8263. doi: 10.1371/journal.pone.0008263

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Linher et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The expression of several marker genes known to be associated with PGCs was determined by real time RT-PCR. Relative quantification of (A) Oct4, (B) Fragilis, (C) Stella, (D) Dazl, (E) Vasa, and (F) c-Kit mRNA levels in D0 undifferentiated skin-derived stem cells and D20, D25, and D30 non-adherent PGC-like cells. Levels were normalized for the RpII housekeeper, and results are presented relative to D30 PGC-like cells. Data represent the mean±SEM of four independent experiments, with different letter subscripts denoting statistical differences between groups (P<0.05).