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. 2009 Dec 14;4(12):e8263. doi: 10.1371/journal.pone.0008263

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Linher et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sodium bisulfite sequencing revealed that the percentage of unmethylated CpGs in (A) undifferentiated skin stem cells at passage 2, (B) undifferentiated skin stem cells at passage 5 (cultured for 25 days in the absence of differentiation media), and (C) D25 PGC-like cells was 81%, 76%, and 99%, respectively. (D) Porcine oocytes served as a control for the expected maternal imprint (98% unmethylated). Open and black circles represent unmethylyated and methylated cytosines, respectively.