Skip to main content
. 2009 Dec 14;4(12):e8263. doi: 10.1371/journal.pone.0008263

Figure 5. PGC-like cells lentivirally transduced with the Dazl-GFP reporter give rise to OLCs.

Figure 5

Bright field and fluorescent images of a (A) representative aggregate and (B) an OLC derived from D25 PGC-like cells transduced with a Dazl-GFP lentivirus. (A) A GFP+ putative germ cell within an aggregate. bar = 100 µm. (B) After further differentiation, a GFP+ OLC was obtained (a, upper panel). OLCs generated from non-transduced PGC-like cells were GFP- (b, lower panel). bar = 50 µm. (C) Representative agarose gel images depicting the presence of Gfp mRNA in transduced OLCs (upper panel on the left). GFP+ OLCs also expressed Vasa, Oct4, and Gdf9b (panels on the right), confirming their germ cell identity. M: DNA marker, -ve: a reaction without RT enzyme.