Table 1.
Experimental STD intensities of (A) UDP-Galf 1 and (B) UDP-[3-F]Galf 4 at different saturation times. STDmax and Ksat were calculated by fitting the data to an exponential equation and STDfit and epitopes (fit) were derived. The respective T1 relaxation times of the ligand at a 100:1 of ligand : UGM are also displayed.
| (A) | Experimental STDa | STDmaxb | ksatb | STD (fit)c | STD epitopes (fit) a |
T1(s) | ||
|---|---|---|---|---|---|---|---|---|
| 0.5 s | 2 s | 5 s | ||||||
| H5U | 71.4 | 69.0 | 56.5 | 11.64±0.51 | 0.412±0.036 | 4.77±0.41 | 70.9 | ---d |
| H6U | 33.6 | 31.4 | 29.2 | 6.03±0.48 | 0.383±0.053 | 2.29±0.40 | 34.0 | 1.22 |
| H1R | 100 | 100 | 100 | 22.46±1.96 | 0.300±0.044 | 6.74±1.07 | 100 | 2.39 |
| H2R/H3R | 67.2 | 56.8 | 49.1 | 8.91±0.46 | 0.521±0.058 | 4.63±0.58 | 68.8 | 1.25 |
| H4R | 52.9 | 39.6 | 38.7 | 6.57±0.37 | 0.534±0.067 | 3.48±0.49 | 51.7 | 1.07 |
| H5R/H3G | 38.6 | 29.2 | 21.3 | 4.27±0.07 | 0.564±0.022 | 2.39±0.09 | 35.5 | ---d |
| H1G | 21.8 | 24.7 | 26.3 | 5.10±0.04 | 0.333±0.048 | 1.68±0.26 | 25.0 | 2.02 |
| H2G | 20.2 | 19.5 | 21.3 | 7.23±2.07 | 0.182±0.066 | 1.30±0.63 | 19.3 | 1.36 |
| H4G | 17.6 | 20.1 | 20.4 | 3.51±0.25 | 0.424±0.057 | 1.47±0.23 | 21.9 | ---d |
| H5G | 25.2 | 21.0 | 16.1 | 3.46±0.28 | 0.501±0.076 | 1.73±0.31 | 25.7 | 0.99 |
| H6G | 31.9 | 17.4 | 9.53 | 2.02±0.08 | 0.912±0.09 | 1.84±0.21 | 27.3 | 0.53 |
| (B) | Experimental STDa | STDmaxb | ksatb | STD (fit) c | STD epitopes (fit) a |
T1 (s) | ||
|---|---|---|---|---|---|---|---|---|
| 0.5 s | 2 s | 5 s | ||||||
| H5U | 65.8 | 67.2 | 61.1 | 13.76±1.02 | 0.362±0.048 | 4.95±0.76 | 70.7 | 1.81 |
| H6U | 41.0 | 36.7 | 33.3 | 6.71±0.34 | 0.422±0.041 | 2.82±0.31 | 40.2 | 1.26 |
| H1R | 100 | 100 | 100 | 22.60±1.56 | 0.314±0.038 | 7.01±0.98 | 100 | 2.53 |
| H2R/H3R | 65.4 | 58.3 | 50.0 | 9.52±0.50 | 0.502±0.057 | 4.76±0.60 | 67.9 | 1.32 |
| H4R | 65.0 | 51.9 | 44.4 | 8.20±0.42 | 0.554±0.059 | 4.51±0.54 | 64.4 | 1.26 |
| H5R | 32.5 | 25.9 | 22.2 | 4.10±0.20 | 0.553±0.061 | 2.26±0.27 | 32.2 | 0.54 |
| H1G | 33.3 | 32.2 | 33.3 | 7.82±0.64 | 0.285±0.037 | 2.19±0.34 | 31.3 | 2.56 |
| H2G | 50.9 | 39.5 | 38.9 | 7.88±0.23 | 0.454±0.077 | 3.55±0.62 | 50.6 | 1.73 |
| H3G | 25.6 | 18.3 | 16.7 | 2.94±0.23 | 0.582±0.105 | 1.71±0.34 | 24.3 | ---d |
| H4G | --- | 30.6 | 33.3 | 6.44±0.60 | 0.505±0.088 | 3.22±0.64 | 46.0 | 1.14 |
| H5G | 34.2 | 32.9 | 27.8 | 5.38±0.30 | 0.502±0.055 | 2.69±0.33 | 38.4 | 1.14 |
| H6G | 39.7 | 24.4 | 16.7 | 2.85±0.12 | 1.061±0.148 | 3.02±0.64 | 43.1 | 0.54 |
a
For ease of comparison, the STD effects were normalized to the H1R intensity, which was set to 100.
b
From fitting the saturation time data to the monoexponential equation: STD = STDmax (1-e(-ksatt)).42
c
Slope of the STD build-up curve at a saturation time of 0.
d
Not determined because of peak overlap.