Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Nov 24;101(11):1900–1908. doi: 10.1038/sj.bjc.6605419

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

MAD2 down-regulation alters cell cycle kinetics inducing anaphase bridge formation. (A) Following MAD2 siRNA down-regulation, the cell cycle appeared highly de-regulated, showing less cells with 2N DNA content (P<0.0001) and more in 4N (P<0.0001). (B) Western blotting analysis of cyclin B1 shows a significant decrease of this protein in the MAD2↓ cells compared with the untransfected and scramble controls. (C) Anaphase bridges were visualised using haematoxylin staining, which is a nuclear staining able to highlight only the nuclear content (panel a). MAD2↓ cells demonstrate a three-fold higher percentage of anaphase bridges compared with the untransfected and scrambled controls (P⩽0.001) (panel b). (D) MAD2 down-regulation also results in a higher percentage of polyploid cells (>4N) than the control lines (P⩽0.001). Flow cytometry data were analysed by Summit 4.2 software. Mean values were compared using the t-test assuming equal variances.