Figure 2. FGF-2 activates PLCγ1 in a mechanism involving PI3K activation.

(A) HUVEC were labelled with [³H]myo inositol for 24 h and then pre-treated with 10 µM LY294002 for 15 min before stimulation with 100 ng/ml FGF-2. Data indicate the total amount of inositol phosphates generated at the indicated times and are expressed as percentages of the basal inositol phosphates. Data are means±SEM of values obtained from 9 independent experiments. *p<0.05. (B) Calcium release from serum starved HUVEC untreated or treated with 10 µM LY294002. Cells were stimulated with 100 ng/ml FGF-2 and 1 mM ATP at the indicated times (arrows). Data are mean of values obtained from 2 independent experiments.