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. 2009 Sep 4;10(11):1585–1598. doi: 10.1111/j.1600-0854.2009.00972.x

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© 2009 John Wiley & Sons A/S

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Reduction of PRMT5 by RNAi results in abnormal nuclear foci containing SMN and coilin in HeLa cells, but not in SH-SY5Y cells. A) HeLa cells electroporated with PRMT5 siRNA duplexes for 48 h labelled with antibodies to SMN and coilin and counterstained with DAPI. Both antigens are found in abnormal nuclear foci (arrows and insets), whether the duplexes are used as a mix (top row) or separately. Bar = 10 μm. B) Immunoblots to confirm the efficiency of depletion of PRMT5 from HeLa and SH-SY5Y cells. Graphs represent pooled data from three experiments, normalized to tubulin signal and plotted as a proportion of the negative control. C) SH-SY5Y cells with lowered PRMT5 show no significant changes in the distribution of coilin and SMN in nuclear bodies (one-way anova with Bonferroni multiple comparison post-test, total n = 632, p > 0.05 for all relevant comparisons).