Figure 5.

Anti-sDMA antibodies immunoprecipitate coilin, SMN and Sm proteins from undifferentiated and differentiated SH-SY5Y cells. Equal amounts of whole cell lysates from undifferentiated or BDNF-differentiated SHSY-5Y cells were used for immunoprecipitation using anti-coilin antibodies and the anti-sDMA antibodies Y12 and SYM10. Duplicate blots of immunoprecipitated material were probed with anti-coilin (top), anti-SMN (middle) and Y12 anti-sDMA (bottom). Endogenous coilin was immunoprecipitated by anti-coilin, Y12 and SYM10 antibodies. SMN was immunoprecipitated by Y12 and SYM10 antibodies, with a very small amount also immunoprecipitated by anti-coilin. SmB/B′ was immunoprecipitated by Y12 and SYM10 but not by anti-coilin. The symbol ’*’ denotes detection of antibodies used for immunoprecipitation.