Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 4;10(11):1585–1598. doi: 10.1111/j.1600-0854.2009.00972.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 John Wiley & Sons A/S

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

PMC Copyright notice

Reduction of SMN expression with RNAi disrupts CBs as well as gems. A) HeLa cells microinjected with siRNA duplexes to reduce SMN expression (demonstrated by red signal in TR-dextran panel, far right, and arrows) lack SMN-containing gems (left hand panel, green on overlays). Antibody detection of coilin (top row, magenta on overlays; bottom row, green on overlays) shows reduced CBs and increased diffuse nucleoplasmic signal. Antibody detection of the non-snRNP splicing factor, SC-35 (bottom row, magenta on overlays), reveals intact nuclear speckles. Bar = 10 μm. B) Undifferentiated (top row) and BDNF-differentiated (bottom row) SH-SY5Y cells microinjected with siRNA duplexes (red signal in TR-dextran panel, far right, and arrows) lack SMN-containing gems. Coilin-containing CBs (magenta on overlays) are also absent. Bar = 10 μm.