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. 2009 Aug 18;18(11):2209–2218. doi: 10.1002/pro.225

Figure 5.

Figure 5

(A) Crosslinking assay of full-length N protein in the presence of various concentrations of glutaraldehyde. The concentration of the protein was 2 μM. (B) Gel filtration chromatography-based analysis (Superdex 300 XK16/70) of N protein dissolved in 50 mM Tris-HCl (pH 7.3), 150 mM NaCl, and 0.1% CHAPS. The concentration of the target protein was 3 μM. Standard proteins of 669 kDa, 158 kDa, 67 kDa, and 43 kDa are denoted as 1, 2, 3, and 4, respectively.