Figure 2.

(A) Full-length amino acid sequence of 2LTRZFP. The amino acids shown by underline were located at the specific positions of −1, 3, and 6 from the N-terminal of the α-helix, respectively. (B) Full-length optimized nucleotide sequence of 2LTRZFP. (C) Primary sequence alignments of Aart (PDB code 2I13) and 2LTRZFP with 80% sequence identity. The underlined letters represent amino acid residues of the ZFP those are different from the Aart peptide. Positions −1, 3, and 6 above the recognition helix column represent amino acid residues often involved in DNA recognition. Numbers at the end of each line show the running number of the last amino acid residue.