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. 2009 Aug 21;18(11):2219–2230. doi: 10.1002/pro.233

Figure 3.

Figure 3

(A) SDS-PAGE analysis of His6-2LTRZFP-GFP at different and purification steps. Lane 1: protein marker, lane 2: total bacterial lysate, lane 3: total bacterial lysate with pTriEx-4-2LTRZFP-GFP after IPTG induction for overnight, lane 4: pass through lysate, lanes 5, 6: solution after washing the column with binding buffer and washing buffer, respectively, lane 7: elution of purified His6-2LTRZFP-GFP. The arrow indicates the size of 50 kDa of His6-2LTRZFP-GFP. Numbers on left show size in kilo Dalton of protein ladder. (B) Western blot analysis of His6-2LTRZFP-GFP at different condition of protein expression. Lane 1: total bacterial lysate, lanes 2, 3: total bacterial lysate with pTriEx-4-2LTRZFP-GFP after IPTG induction for 4 h, and overnight, respectively. The arrow indicates the size of 50 kDa of His6-2LTRZFP-GFP. Numbers on left show size in kilo Dalton of protein ladder.