Figure 5.

Kinetic analysis between 2LTRZFP-GFP and its target DNA sequence. (A) Binding kinetic of 2LTRZFP-GFP (300, 200, and 100 nM), GFP, and BSA to the immobilized chip with specific ds-DNA. (B) For the competitive SPR, 2LTRZFP-GFP was incubated with nonbiotinylated ds-DNA of its target ds-DNA, and nonspecific ds-DNA in zinc buffer for 15 min before injection. (C) 2LTRZFP-GFP was injected to determine the binding activity with its target DNA sequence in zinc buffer containing 1 μM, 1 mM, and 10 mM of EDTA.