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. 2009 Aug 21;18(11):2219–2230. doi: 10.1002/pro.233

Figure 6.

Figure 6

Electrophoretic mobility shift assay (EMSA). (A) The protein used for each reaction is 1 μM, whereas [0], no DNA duplex; [+], specific DNA duplex; [−], nonspecific DNA duplex. The gel was stained with SYBR® Green EMSA stain (B) or with SYPRO® Ruby EMSA stain (C). In panels B and C: lanes 1 to 9, the specific DNA duplex used for each reaction was 250 nM, whereas 2LTRZFP-GFP was varied, the concentration from 0, 0.125, 0.25, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 μM, respectively. Lane 10, 2LTRZFP-GFP was used 3.0 μM, but no DNA duplex.