Table I.

Purification of hEpman from 2.3 L of Culture Medium of Stably Transfected Cells^a

Fraction	[Protein], (mg/mL)	Total protein (mg)	Total enzyme (units)	Specific activity (U/mg)	Yield %	Purification fold
Culture supernatant	2.2	4945	24.1	0.0049	100	1
Cibacron blue	0.1	112	17.8	0.16	74	33
Chelating Sepharose	0.6	45.0	15.6	0.35	65	71
Cation-exchange
Peak 1	2.4	11	5.3	0.50	22	102
Peak 2	4.3	32	15.6	0.49	65	100
Superdex 200
Peak 1	19	10	6.3	0.66	26	135
Peak 2	16	25	22	0.9	91	182

^aEach step in the purification was assayed using 3 mM pNP-Man as substrate. Activities fluctuate slightly as the enzyme was inhibited by salt present in the elution buffers.