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. 2009 Aug 31;18(11):2252–2264. doi: 10.1002/pro.236

Table III.

Summary of the Point Mutant Proteins Used in This and Other Studiesa

Residues MCM binding Re-replication
K441A, K445Ab NDc
P504T,E508A,H510A,L514Ad +
R465A,E468A,L469A,A470S, R471Ae +
R368K,P467H,L473E,V476A, F477Af +
R481S (K543S)g + +
K482S (K544S)g +
T486A (T548A)g + +
R481S, K482S, T486Ah -
S523Ag + +
H525Sg + +
R528Sg + +
S523A, H525S, R528Sh
V512Ag + ND
L513Ag + ND
E516Ag + ND
a

Residues number is based on mCdt1.

b

From You and Masai.17

c

Not determined.

d

Originally residues of human Cdt1 (hCdt1) were mutated, but mCdt1 residues equivalent to hCdt1 (P492T, E496A, H498A, L502A) were listed for convenience.27

e

mCdt1 residues equivalent to hCdt1 (R453A, E456A, L457A, A458S, R459A) were listed for convenience.27

f

mCdt1 residues equivalent to hCdt1 (R356K, P455H,L461E,V464A, F465A) were listed for convenience.27

g

Mutations from this study. Residue number inside parenthesis is for the Xenopus Cdt1.

h

Mutations from this study. Both mCdt1 and Xenopus Cdt1 mutant proteins were used.