Table III.
Residues | MCM binding | Re-replication |
---|---|---|
K441A, K445Ab | − | NDc |
P504T,E508A,H510A,L514Ad | − | + |
R465A,E468A,L469A,A470S, R471Ae | − | + |
R368K,P467H,L473E,V476A, F477Af | − | + |
R481S (K543S)g | + | + |
K482S (K544S)g | − | + |
T486A (T548A)g | + | + |
R481S, K482S, T486Ah | − | - |
S523Ag | + | + |
H525Sg | + | + |
R528Sg | + | + |
S523A, H525S, R528Sh | − | − |
V512Ag | + | ND |
L513Ag | + | ND |
E516Ag | + | ND |
Residues number is based on mCdt1.
From You and Masai.17
Not determined.
Originally residues of human Cdt1 (hCdt1) were mutated, but mCdt1 residues equivalent to hCdt1 (P492T, E496A, H498A, L502A) were listed for convenience.27
mCdt1 residues equivalent to hCdt1 (R453A, E456A, L457A, A458S, R459A) were listed for convenience.27
mCdt1 residues equivalent to hCdt1 (R356K, P455H,L461E,V464A, F465A) were listed for convenience.27
Mutations from this study. Residue number inside parenthesis is for the Xenopus Cdt1.
Mutations from this study. Both mCdt1 and Xenopus Cdt1 mutant proteins were used.