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Thermodynamic Values at pH 8 and pH 5
| pH | ΔH°NUa (kcal/mol) | ΔS°NU (e.u.)b | TM,NU (K)c |
|---|---|---|---|
| 8d | 23.8 ± 1.6e | 76.9 ± 5.1 | 309.7 |
| 5 | 23.7 ± 1.6 | 76.5 ± 5.1 | 310.2 |
NU = native to unfolded, temperature ramp from 5 to 60°C.
e.u. expressed in cal mol−1 K−1.
Tm is calculated according to Eq. (9).
The concentrations of domain 4 for measurements at pH 8 were 12.1 μM, 67 mM NaCl; for pH 5, the data are values recorded at 5 μM, 140 mM NaCl.
Errors determined from the fit to Eq. (8).
