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. Author manuscript; available in PMC: 2009 Dec 3.
Published in final edited form as: Biochemistry. 2007 Oct 4;46(43):12212–12219. doi: 10.1021/bi701496z

Fig. 5. Effect of varying CPR levels on WT, R254K, and sHO1.

Fig. 5

Rate of bilirubin formation was monitored in reactions containing 0.1 nmoles of HO-1 and CPR levels ranging from 0uM to 0.4uM. The homogenous full-length HO-1 (R254K) exhibited an increased activity and binding affinity for CPR, in comparison to the WT and sHO-1 enzymes. WT HO-1 had a slightly higher overall activity than the sHO-1.