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. 2009 Dec 1;23(23):2723–2728. doi: 10.1101/gad.1812609

Figure 2.

Figure 2.

ULT1-dependent expression of phenotypes caused by AG ectopic activation. (A–C) clf-2 phenotypes are rescued in an ult1-3-null mutant background. (D) RNA blot of rosette leaf tissue showing reduction in clf-induced ectopic AG expression in 10-d-old clf-2 ult1-3 plants. The relative amount of AG transcript accumulation is indicated, normalized to rRNA. (E–G) RNA in situ hybridization showing expression of AG in stage 3 floral buds of ult1-3, clf-2, and ult1-3 clf-2 plants. (H–J) Loss of ULT1 rescues 35S∷AG phenotypes in a dosage-dependent fashion. Plants shown are 35S∷AG transgenic in a wild-type (WT), ult1-3/ULT1 heterozygous (ult1-3/+), or ult1-3 homozygous (ult1-3) background. (K) RNA blot of rosette leaf tissue showing ult1-induced reduction of ectopic AG expression in 28-d-old 35S∷AG plants. The relative amount of AG transcript accumulation is indicated, normalized to rRNA. (L) RT–PCR with leaf RNA of 28-d-old plants. Primers were used to detect total AG mRNA, AG mRNA from the 35S∷AG transgene, AG mRNA from the endogenous locus, AP3 mRNA, and SEP3 mRNA. EF1α is shown as the reference gene. Identical results were obtained in experiments using the independent ult1-1 and ult1-2 alleles.