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. 2009 Dec 1;23(23):2717–2722. doi: 10.1101/gad.1851809

Figure 5.

Figure 5.

RDM4 interacts with NRPE1 and RPB1. (A) The RDM4 protein level was assessed by Western blot analysis in the indicated genotypes. A nonspecific band (Control) is shown as loading control. (B) Coimmunoprecipitation between RDM4 and NRPE1-Flag. The protein extracts from the indicated genotypes were precipitated by anti-Flag antibody-conjugated beads, and the precipitates were detected by anti-RDM4 and anti-Flag antibodies. (C) Coimmunoprecipitation between RDM4 and RPB1. The protein extracts from ros1 and ros1rdm4 were precipitated by anti-RDM4 antibody-conjugated beads, and the precipitates were detected by anti-RPB1 and anti-RDM4 antibodies. (D) The protein extracts from wild-type and YFP-RDM4 transgenic plants were precipitated by anti-RPB1 antibody-conjugated beads, and the precipitates were detected by anti-YFP and anti-RPB1 antibodies.