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Most of the nuclear Stu1 associates firmly with detached KTs. (A) Stu1 polymerizes at detached KTs. Stu1 was overexpressed from a GAL promoter in a wild-type STU1 background during a G1 arrest. Cells were analyzed 240 min after the release from G1 into nocodazole. (B) Stu1 localization at detached KTs is stable. FRAP analysis of Stu1-GFP. (C) Stu1 localization at metaphase spindles is dynamic. FRAP analysis of Stu1-GFP in cells arrested in metaphase by Cdc20 depletion.
